Hello everyone, it seems my virus protein can be degraded by the host via ubiquitination. I want to search for which E3 ligase is responsible for this. Do you have any suggestions? Thanks!
I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...
13 August 2024 9,795 0 View
In the Itasca FLAC3D Documentation, it provides the expression of plasticity multiplier regarding Mohr-Coulomb criterion directly, without a detailed derivation process. Could anyone share the...
12 July 2024 7,850 0 View
I am conducting a study on sandstone samples and need to accurately differentiate between tuffaceous matrix and clay matrix when examining thin sections under a polarized light microscope. While...
20 June 2024 3,270 0 View
It is generally believed that time dilation and length contraction is a geometric effect, is there anyone has tried to explain the time dilation and length contraction effect in a geometric...
05 June 2024 1,591 6 View
Delay could induce the periodic behaviors through Hopf bifurcation, Are there any other bifurcations induced by delay that can lead to periodic behavior? Are there some references?
16 May 2024 10,003 1 View
The equation in fig.1 can be transformed to the one in fig.2. Using ‘atan’, we can turn the periodic function ‘tan’,which is on the left of Eq.1, to the sum of ‘atan’ and l*π. However, for the...
14 May 2024 8,585 4 View
Tansverse resonance condition for the single layer(FIg.3) waveguide has bee deduced, as shown in Fig.2, which contains the phase shifts cause by reflection and optical path difference. Only light...
24 April 2024 1,748 4 View
Thank you!
10 April 2024 8,336 1 View
In my experiment, I need to add three monomers to the solvent. Adjust the content of different monomers to obtain the materials I need. Now, I have a rough idea of what the polymer content is for...
06 April 2024 9,568 2 View
As the article Ligands for CsPbBr 3 perovskite quantum dots: The stronger the better? reported, Reaction Yield= mpurified*w%/n*MPb where mpurified is the mass of the dried quantum dots, w% is the...
01 March 2024 8,981 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View