Narmadha Murugesan

3 Questions 5 Answers 0 Followers

Questions related from Narmadha Murugesan

How to get good positive peak in single stain controls for rare cell type markers in flow cytometry?

What to do if single stain control using endogenous reporter do not show good positive peak

19 July 2025 7,956 3 View

What is the optimum cell density to transduce C2C12 cells with lentivirus? what does cause poor transduction efficiency?

I am not able to transduce C2C12 cells with lentivirus-GFP. I seeded 75,000 cells in a 6 well plate and I transduced after 24hrs. But they become too crowded at 48hrs and did not show GFP+ cells

04 February 2025 6,868 4 View

How to validate my flow cytometry protocol for quantifying muscle satellite cells?

I am using CD45,CD31,Sca1,PDGFRa (negative selection) and a7Inegrin+ as panel to quantify satellite cells. But when I validate this with Pax7CreERT2;tdTomato mice(which labels satellite cells with...

04 February 2025 7,603 3 View