How to use udec to simulate acoustic emission phenomena?

01 June 2023 0 4K Report

How to monitor AE signals detected by laboratory coal samples and microseismic signals in field mining engineering in numerical simulation?

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How to make ppb level concentration ?

Hi, I've N2 gas cylinder and 100 ppm NH3 gas cylinder. With these two cylinders, I'd like to make a ppb level (1- 100) concentration. Any idea how to do that?

01 February 2021 3,264 2 View

What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

11 January 2021 8,775 10 View

How to increase the attachment of the HEK293t cells to the glass coverslips or cell dishes?

Hey guys, I always have problems with the attachment of HEK293t cells to glass coverslips while doing IFA. They are so easy to detach from the coverslips while washing. The coverslips are already...

30 December 2020 8,381 3 View

Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

Looking for a glass slide with very low Autofluorescence (GFP)?

Does anyone know any glass slides (thickness 0.1 to 1mm) that have very low autofluoresence (GFP). I am doing a very sensitive experiment that require my glass substrate to be as low fluoresence...

28 November 2020 1,917 6 View

Function of SDS in Northern Blot washing step?

In Northern blot, we normally use either 2*SSC+0.1% SDS or 0.1*SSC+0.1% SDS in washing step. Does anyone know what's the function of the SDS in the washing buffer? Thank you in advance

26 November 2020 2,432 1 View

How to calculate the analytical solution for the HYDRUS-3D model simulation of water and salt movement in soil?

Suppose an agricultural field with uniform soil texture and salinization. The groundwater depth is 20 meters, assuming that the depth of the groundwater and the concentration of salts remain...

25 November 2020 2,382 4 View

Protein concentration decrease after dialysis?

Recently I have been involved in this problem: After expressed by adding IPTG, lysed with B-PER, and resolved with Buffer B (8M urea), incubate with Ni-NTA beads, everything looks fine. When I...

21 November 2020 7,416 3 View

Recommend equipment of in vivo recording in mice brain?

Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!

12 November 2020 2,508 1 View

Looking for a method that aggregate Nano beads on demond?

I am working on a droplet based digital biosensing project. Assume I have some droplets (size 30-40um in diameter), each droplet contians aroun 10-20 Nano beads (100-300nm in diamter). The surface...

28 October 2020 5,761 7 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View