How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

How can we test interaction effect using repeated measure design?

Hi! I am currently working on a problem using repeated measure design. Each participant was assigned to two CONDITIONs (A and B). And their GOAL (continuous) were measured twice in condition A and...

25 November 2020 1,235 3 View

When performing mutliple behavioural tests over a period of time, should you habituate the animals before every test or just once at the beginning?

I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is...

17 June 2020 9,083 5 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

Can I use satellite images to detect the leaking and clogging for a Surface Drip Irrigation System?

Hello everyone: I have being searching for three weeks now to find a possible solution using remote sensing. This application is for a vineyard 200m by 300m or even bigger. It has a a surface...

28 February 2020 3,723 5 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

05 February 2020 1,232 3 View

How to check the quality of MgO for magnetic tunnel junction?

I am making some magnetic tunnel junctions, but I always get the junction shorted. I am not sure whether the quality of MgO is good. Does anyone know how to check the quality of MgO? Such as AFM...

28 January 2020 139 2 View

Implement Topshim (Bruker NMR) shimming commands in IconNMR?

With Bruker's Topshim program, I have used the following command to shim every new sample: topshim z6 1d optsolvent coil=1H tuneb tunea Now I'd like to use IconNMR to automate this procedure,...

20 August 2019 1,111 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

How to mesh in crack tip using shell element in ABAQUS?

S8R elements were applied in 2D cracked plate in ABAQUS. But when I drew a circle contour in the crack tip, it showed an error. I also checked the ANAQUS Manual and it told me that 'Focused...

21 April 2019 3,698 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View