I am asking for technical details of the SEC column, including the type of column used, the standard material, any protocol that could help in this issue. What are the critical points in terms of instrumental settings (HPLC and ICP-MS). Do we need guard column or not? The buffer phase (eluent). Also, what is the best Humic acid that can be used in such experiments (SNOM or any suggestions)?
Thanks Zheng. I am looking for more details. A help from expertise will be appreciated . Thanks
Dear Ezzat,
the same authors as Zheng suggested present their method in more detail here:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164845/
Hope it helps
Regards
For some tips, we do not use the guard column, but you need to filter your sample before injection. Any mobile phase can be used in the SEC, even pure water. You may need some standards to indicate the molecular weights of peaks.
Thanks Zheng, Ok, what about the standard of different metals. I need to detect the concentrations of metals associated with different MW. How I can add the standard? I need something that is stable in the mobile phase and that can make it through the column to give a single integrate-able peak. For example, it is doubtful that standard Cu (as a good candidate) in solution would make it through the column at pH 7 (as most mobile phase in such work are alkaline) without getting adsorbed and I will get nothing in the ICP-MS (no peaks). Any suggestion, Thanks again
It is a little strange that there is no peak when the Cu solution is injected. I never try the quantitative method for SEC-ICP-MS, because it is difficult to get good peak shape for calculating the peak area when using natural samples. Just a suggestion,you may try to use EDTA-Cu or other chelated Cu as standards.
It may be more realistic to inject Cu into the effluent stream post SEC column. This would give you a calibration curve for Cu concentration without having to worry about adding chelating agents
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