How to use protein sequences in docking?

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Does Graphene Powder really exist?

It is said that graphene layers accumulate again if they are produced and are not in a suitable solvent. So how are these pristine graphene powders supplied? I do not mean graphene oxide or...

29 May 2024 378 1 View

I have a solution containing sodium hydroxide salt and graphene. What is the best way to separate the two?

My suggestion is phase transfer or using a dialysis bag. Is there any way to precipitate sodium hydroxide?

17 April 2024 8,808 3 View

Is it possible that a protein Fasta file contains its anti-body also?

I have a protein sequence file, and I noticed that there is a sequence in its Fasta file that is related to one of its antibodies. I want to do a docking between the antibody and the protein. Does...

15 September 2023 9,345 16 View

How can I increase the yield of separated IELs from mouse's colon?

I tried 3 times shake in HBSS and then used 30% percoll and gradient centrifuge with 40% and 70% percoll. But I need to increase the yield of IELs. Does anyone apply a different protocol?

12 September 2023 5,216 0 View

Antimicrobial peptides (AMPs) increase levels of pro-inflammatory cytokines through what mechanism?

In IBD , AMPs how or through what mechanism increase expression of inflammatory cytokines? Could someone please give me some advice on this?

16 July 2023 7,191 2 View

What is the reason for Ct difference greater than 0.5 between duplicate samples in PCR ?

Hello, I did PCR for mouse genotyping; the Ct difference for around half of my duplicate samples was greater than 0.5. I cannot find what the problem is. If someone could assist me with this...

24 May 2023 1,752 3 View

How I can research on early childhood mathematics education? How I can start ? It needs to go to university and study in this field?

I was a math teacher for a long time. I have done MS in math education . Now I am studying ECE. please guide me if it is possible.

16 May 2022 3,553 8 View

How can I get the bibtex file of refernces in a paper?

For writing a thesis, I should use some refernces in a paper. Is there any way to download the bibtex file of refernces? In Springer website, there is a link to download the ris(it can be...

16 March 2021 2,518 4 View

Ester formation from COOH and OH by using EDC/ NHS is possible?

Ester formation from COOH (Carboxylic acid ) and OH(alcohol) by using EDC/ NHS is possible؟

13 October 2019 8,958 4 View

How should I select the meaning unit in Qualitative Content Analysis correctly?

Below is a segment of Satu Elo et al. article (Qualitative Content Analysis: A Focus on Trustworthiness) : It is important to fully describe the meaning unit when reporting the analysis process so...

01 July 2015 8,480 10 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View