I am trying to work with calibration curve to find the cysteine residues on GFP
Cysteine is incubated with 0.5 mM DTNB for 60 min at room temperature - Absorbance read near 412 nm - Neutral or alkaline pH
http://dx.doi.org/10.1016/0003-9861(59)90090-6
Hope this protocol from thermoscientific helps you out.
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