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30 August 2020 2,230 5 View

How to get pure recombinant virus with plaque purification ?

We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...

01 August 2020 2,087 1 View

How to make Sentaurus silver.par to be the perfect conductor like defined electrode?

I have been trying to create some perfect conductor in my simulation as a connection between 2 spots(different doping semiconductor area). But is seems that the barrier bewteen silver and the...

10 March 2020 2,535 1 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

What is the best indoor spectrum that people need?

What is the best spectrum for an office, classroom, or other architectural scenes? Spectrum affects people's visual needs, work efficiency, comfort, psychological or physiological rhythm. How to...

20 December 2019 5,715 4 View

What effect does precipitation pH and heat treatment temperature have on the electrical resistance of the material?

I have prepared a series of nanomaterials by co-precipitation under different pH value and heat treatment temperature. I want to find a reasonable explanation about the change of the material...

21 August 2019 9,192 3 View

Sentaurus simulating anti-reflection coating issue?

I am starting to simulate the anti-reflection coating (ARC) for solar cells but I found some problems that I can't answer: 1, For solar cell without ARC, I tried to put the light source below...

03 July 2019 6,682 2 View

How to consider the question of theoretical support for statistical model choosing?

In my research I focus in paired correlated binary data. To accommodate the intraclass correlation, I choose to adopt a certain model without providing much theoretical support, and was criticized...

04 June 2019 7,887 12 View

does anybody have DR5::GUS vector to share?

does anybody have DR5::GUS vector to share? i need to check auxin in potato. thanks a lot.

03 March 2019 3,449 6 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View