HI, I've started ti learn using JMRUI to analyse NMR spectroscopy signals. I completely don't know how to use AMARES quantitation or any other type of quantitation. I have to determine the concentration of metabolites in the spectrum derived from the human brain. I have watched tutorials on youtube, but during them, there are used some databases .sv type to make quantitation and I don't where can I find something like that?
I really beg for help
Best regards,
Aleksandra
The time domain quantification of metabolite signals was conducted using AMARES algorithm with custom prior knowledge.Metabolite concentrations were reported for tCr (creatine plus phosphocreatine), NAA (N-acetyl-aspartate), tCho (phosphocholine and glycerophosphocholine), Ins (myoinositol) and Glx (glutamate and glutamine). The AMARES prior knowledge model consisted of peaks for NAA, choline (Cho), creatine (Cr), glutamate + glutamine (Glx) and myoinositol (Ins). The amplitudes of NAA, Cho, Cr, Glx, and Ins peak were estimated by the algorithm. The relative phases of NAA, Cho, Cr, Glx, and Ins peak were fixed at 0. The linewidth of NAA was estimated by the algorithm, and the linewidths of the remaining peaks were set to be equal to that of NAA. The frequencies of NAA, Cho, Cr, Glx, and Ins peak were estimated by AMARES. All peak shapes were fixed at Lorentzian.
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