The cause of blockage is deposition of either dried blood or dye particles from microinjection. Unfortunately, immersing the needles in 100% ethanol and flushing them through ethanol, using the hamilton syringe did not unclog it.
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
We have no access of pressure plate apparatus
22 July 2024 3,488 2 View
give suggestions
22 July 2024 5,664 2 View
Since BET and TGA both are very costly instruments and many places the facilities are not available (for large number of samples) hence a threshold degassing temperature and time is preferable
16 July 2024 8,112 2 View
In the peptide-HLA-TCR docking process, can we express the result of peptide-HLA docking in RMSD and for peptide-HLA to TCR docking, can results be expressed in binding affinity?
11 July 2024 4,831 0 View
No data regarding radius of influence is provided and no pumping test data available.
10 July 2024 7,168 0 View
I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...
01 July 2024 3,267 1 View
I need to apply an external electric field to 4-hydroxybenzoic acid to compare the molecule's reactivity descriptors. Could you guide me on how to apply the electric field using Gaussian and...
01 July 2024 4,192 3 View
I'm trying the edit the orientation of some side chains in a homology protein model I made. Can I use PyMOL for such tasks? If so, could anyone navigate me to some online tutorials? If not, what...
01 July 2024 4,409 4 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP
09 July 2024 6,038 5 View
What is safe gasoline and Ethanol safe or Ethanol petrol mixture safe for car fuelin.
03 July 2024 2,939 3 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...
17 June 2024 4,183 3 View
Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...
16 June 2024 2,754 0 View
Anyone know of a good replacement for the needle used to puncture gradient tubes on the brandel gradient fractionator? We use this system to fractionate sucrose gradients for polysome profiling,...
06 June 2024 8,323 0 View
We are trying to use 100% ethanol as a precipitant for Bovine Serum Albumin. The BSA is in a 0.5% concentration and we are using various ratios of ethanol to attempt to precipitate it, such as 1:2...
04 June 2024 8,024 1 View