I would like to create a wound on porcine skin and then seeded MRSA bacteria for growing biofilm. However, when I cultured for 2 days, I found many other bacteria on the agar plate.
Does anyone know how sterile porcine skin before create wound? I used to to immerse the porcine skin for 30 min. I also tried 2 h and 4 h, and it did not help at all.
Kindly check the following links that may be helpful:
Development of a High-Throughput ex-Vivo Burn Wound Model Using Porcine Skin, and Its Application to Evaluate New Approaches to Control Wound Infection:
Article Development of a High-Throughput ex-Vivo Burn Wound Model Us...
Bacterially sensitive nanoparticle-based dissolving microneedles of doxycycline for enhanced treatment of bacterial biofilm skin infection: A proof of concept study:
Article Bacterially sensitive nanoparticle-based dissolving micronee...
Evaluation of short exposure times of antimicrobial wound solutions against microbial biofilms: from in vitro to in vivo:
https://academic.oup.com/jac/article/73/2/494/4641704
Dear Dr Fan Zhao . See the following useful RG link: Article Cadexomer iodine effectively reduces bacterial biofilm in po...
Kindly check also the following very good RG link: Article Bacterially sensitive nanoparticle-based dissolving micronee...
Also please check the following very useful RG link: Article Candida auris Forms High-Burden Biofilms in Skin Niche Condi...
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