How to track fluorescent proteins inside bacterial cells?

24 August 2017 1 1K Report

I am trying to track a bacterial DNA binding protein during its cell cycle. I have phase contrast image of the cell and the protein tagged with GFP. I want to track my protein inside cell in time-lapse experiment. Is there any FIJI/ImageJ plugin that can work with both phase contrast and fluorescent images together? Thanks in advance

Ramachandramouli Budida

The single molecule tracking technique helps in your case. Please find the following links that might help you. The link 1 should be more close to your case. Goodluck

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144692/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934628/

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