hello everyone , i am very new to regcm4 ,could not figure out how to run the model in a nested way from the namelist also what are available data sets we can use for initial and boundary conditions?
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...
06 June 2024 2,289 3 View
I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...
03 June 2024 9,767 0 View
I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...
24 May 2024 8,549 1 View
Optimal power flow, small hydro
20 May 2024 7,590 0 View
I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...
18 May 2024 4,289 3 View
I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?
25 April 2024 5,386 1 View
explain its morphology, taxonomy and habitat distributiion
03 March 2024 1,293 1 View
Suppose we are performing a phylogenetic analysis among different fungal species and we wanted to see how closely our species of interest is related with the other species across different...
28 January 2024 9,895 4 View
I want to establish a phylogenetic relationship of our fungal species of interest along with the other fungal species belonging to the same order (Hypocreales). In order to perform this, we have...
22 January 2024 9,174 5 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...
24 July 2024 8,172 1 View
Hello everyone, I am conducting
22 July 2024 8,085 11 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View