I want to analyze the Ni NPs in earthworm’s tissues.I am not sure which part of earthworms may contain the NPs. Should we go for TEM for testing this? If yes, than how we should select the sample for tissues? Is there anyone who used the confocal microscope for testing the NPs in biological tissues? How we can prepare the dyes for this experiment? Is that confocal microscope is good choice as compared to TEM/SEM? Your help will be highly appreciated if someone can provide me working protocol.

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