Is there any method to confirm that the CT-DNA is fully dissolved in buffer? This is because the mass to volume ratio i added CT-DNA into buffer ended up with a concentration much lower than i expected from what i read online.
I am seeking advice on the matrix and solvent conditions.
29 May 2024 2,360 0 View
I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...
27 April 2024 3,077 0 View
Hi all, I'm Dabin Xie from Zhincai Tech, a small research-driving company in China. I'm currently trying to modify glass with a single layer of silane whether hydrophobic or hydrophilic, like...
08 April 2024 2,628 4 View
How to solve this problem when Aspen economic evaluation was performed? It said "Temperature difference is insufficient to perform the heat transfer."
06 April 2024 7,291 3 View
If I want numerical simulation software to simulate superconducting magnetic shielding, which software is better for simulating superconducting magnetic fields?
25 March 2024 5,589 1 View
I've encountered an issue following an LR reaction intended to construct a gene within a pENTR-3C (KanR) vector and combine it with a pCDH (AmpR) vector. Despite expectations, the protein was not...
14 February 2024 3,502 0 View
I tried to make a moive of protein structure in PyMOL. However, when I stored the movie for future use, the PyMOL reminded that the encoders were not installed. How to install these encoders in...
29 October 2023 5,129 2 View
I have performed the 3C-qPCR assay and designed the primers of interested region, but I do not have BAC clone as control. How should I normalize the prime efficiency and background noise?
16 October 2023 4,387 0 View
In the XRD experimental analysis of polycrystalline TiN films, are TiN (111) and TiN (222) different crystal planes? Or are they the result of different diffraction levels of the same crystal...
26 September 2023 3,580 5 View
If a protein was designed as a closed cyclic protein (N-and C-terminus closed after reaction), can PyMOL show this structure? Because in PyMOL, the sequence is always linear, so how the closed...
17 September 2023 2,886 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View