Does anybody can share the protocol on taking the skin lesions from horses for Leishmania detection ?
Diagnosis of cutaneous leishmaniasis in horses is made by impression smear or biopsy of the lesion with protozoa identified within macrophages in stained smears. Polymerase chain reaction (PCR) and sequence analysis can be used to confirm the diagnosis and identify the species. PCR targeting the internal transcriber spacer 1 (ITS1) is the most sensitive. Serology tests are unreliable, horses can have antibodies from a previous (silent?) infection.
Dear Dr. Appeared,
Thank you very much for your answer. I was interested in procedure itself, how to take a skin lesion biopsy, whether anesthesia is required during the procedure, and the dosage of course. Could you please provide me with this details ?
If you take an impression smear from the lesion I'm sure no anesthesia is needed. For a regular biopsy anesthesia is required (also for your own safety) but usually you get bad anesthesia in inflamed areas. Perhaps a "spray" anesthetic is sufficient?
It was urgently needed for my project and thank you very much indeed for help !
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