Why do cell sensitivity to antibiotic resistance increase after lentiviral infection?

03 November 2020 7,754 2 View

How to calculate the capacity of a battery from electrodes capacity?

I found that 1/Q = 1/Q+ + 1/Q- for specific capacity, I will explain my problem. Let's imagine that my positive electrode is 300 mAh/g my negative electrode capacity is also 300 mAh/g If i do a...

26 August 2020 568 3 View

Does cytotoxicity measure vary at same molecule concentration but different cells concentration ?

I have made a cytotoxicity assay using MTT at 20K cells/well in a 96 wells plate. If I do a MTT assay at 50K cells/well and at 10K cells/well, will i found the same effect ? I tried to find some...

07 April 2020 1,901 4 View

What are the affordances of video to research and practice in education?

I'm interested in digging deeper about the question : What are the affordances of video to research and practice in education? Please feel free to suggest a good paper on this theme. If it is...

27 November 2019 4,010 4 View

How to build an optimal computer for DFT calculation at a reasonable price?

I would like to build my computer from the beginning, part by part, in the purpose of DFT calculations and computational chemistry. I never did that and I'm kind of a noob in computer science. So...

22 April 2019 840 4 View

Why transformation of diploid strains in yeast is less efficient than haploid ?

Hello, For some time I realised the diploid w303 wt strain I was using was less efficient in the number of transformants I was getting. So I did a small transformation control with two different...

16 October 2018 7,486 2 View

What's the physical structure of space-time ?

A. Einstein in 1920 realized that his theory might not be complete, in the sense that Mach's principle was not consistently applied to the general relativity theory as space-time is not made of...

26 June 2018 3,196 51 View

Where does this information come from?

from uniprot : Nedd4 binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif.

25 July 2017 9,694 1 View

Chi Square Unequal sample Size?

15 September 2016 6,243 7 View

Which IHC marker can we use to differentiate gastric cancer from normal gastric tissue ?

I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...

24 August 2016 8,716 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View