How to study apoptosis in ischemic stem cells?

09 September 2019 1 2K Report

I am working on ischemic model of hDPSCs. I want to study apoptosis in the cells challenged for different periods of ischemia (3, 6, 12, 24, 48, and 72 h). I am following standard protocols of PI and AO/EtBr staining for the study however not getting the expected results. The bright field imaging showed toxicity in the cells but not in PI and AO/EtBr stained cells. Kindly give your valuable suggestions.

Subhash C. Juneja

I have done on tissues paraffin sections by Apoptag kit. Since you have cells and if you have flow cytometry facility, that kind of procedure will suit you.

https://www.bio-rad-antibodies.com/apoptosis-flowcytometry.html

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