please refer this easiest and practical ways

http://sipav.org/main/jpp/index.php/jpp/article/view/1081/864

Dear Sadia Mehdi

Pls. find the attached files, storage for different propose, may help in your research, good luck

http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.2001.91.6.527

https://www.apsnet.org/publications/phytopathology/backissues/Documents/1983Articles/Phyto73n02_241.pdf

http://femsle.oxfordjournals.org/content/204/1/101.abstract

https://www.noble.org/Global/medicagohandbook/pdf/InoculationGrowth_FoliarPathogenic.pdf

http://www.alliumnet.com/images/Onion%20Botrytis%20Diagnostic%20Guide%20-%20PHP%20Nov%202007.pdf

http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-11-266

Regards

Prof. Houda Kawas

Dear Sadia,

The best is to store it at -80C in glycerol 10%. The fungus remains viable for several years.

best regards

Wassim

Hello Sadia, You can use the 10-20% solution of glycerol in distilled water or sterile distilled water to preserve the spores of any fungus for long time. I had used this techniques.

In Addition you can use sterile dry soil for storing the fungal spores. OR slants covered by mineral oils may be helpful.

44

Hi Sadia, I agree with Dr.Vikas, you prepare 10-20% solution of glycerol in sterile distilled water then keep in -80 C

does any one know how to invigorate Botrytis mycelial growth PDA after transferring from glycerol stock. we experienced very slow growth

@Suha Jabaji it takes quite a while for a fungus transferred from a glycerol stock to start growing normally.