I have lyophilized the powdered plant leaf for metabolomic analysis is this method is appropriate for analysis? Does it is also suitable for long time storage?
Lyophilized plant leaf material should be quite stable. For long term storage I would consider a place fresh and dark, or if you want to be sure, frozen to slow down degradation processes that could still happen in your tissue.
My expereince tells me that for metabolic profiling purposes the best option for an accurate analysis is to use the plant material fresh. If one has to work with experiments with large dimensions and numerous samples that cannot be handled easily, I recommned speedVac concentration over lyophilization as there are changes in the metabolites during lyophilization. No matter which technique is used for plant material storage, no longer than a month of deep freeze storage is not recommended.
You can check the results for yourself by spliting a sample in three: no treatment (NT), lyophilized (L) and Speedvac concentrated (S) with 4 stoarge times [ F=fresh, 15, 30 and 45=15, 30 and 45 days storage]. chech the results and use NT-F as reference.
You can work with lyophilized samples, but they should be stored at -80 degrees until the analysis and storage time should be kept low.
Thank you very much to you all for your valuable suggestions,,,
For metabolomics analysis, we usually freeze our samples first in liquid nitrogen prior to grinding them to quench any activities in the tissues. Then we store our samples in -80degC freezer. Hope this helps :)
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