Dear everyone,
Now, I have 1*10^7 sequencing reads (each length < 300nt) for one DNA sequence (~1000nt), but with different random mutagenesis. That means these reads are from many different sequences (~1000) with about %85 similarity and the same length. And, my question is how to splice these reads to obtain the real full-length sequences.
Thanks very much!
Best wishes,
Ya-Zhou
You are talking about the raw data, which is not used for analysis and what you want to do.
Do some kind of preprocessing, trimming, QC and assembly before you reach a point where you can do more specific analysis.
Dear Abhijeet Singh，
Thank you very much for your answer. Yes, you are right. I did talk the raw data, and I want to know how to assemble the sequencing reads. My trouble is that the reads are from many different sequences rather than (the usual) one.
Your best bet might be to try different assemblers with relatively high k-mer values (that will have to be optimized)
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