I'm directing an end-of-degree paper for Pharmacy. It is a question of mobilizing by the electrokinetic process of some dyes and drugs present in natural soils (perfectly characterized). The experiment in its development is simple except in the "analytical part".
Once I have fragmented the contents of the cell into 5 discs, I suspend them in water at the rate of 10 grams of soil per 100 grams of water. I shake it for a day or two and centrifuge it, filter it through normal filter paper, then re-centrifuge it and then filter it again with ultrafine filter paper.
After all this it would seem easy to use visible-ultraviolet spectroscopy to determine the absolute or relative amount of dye or drug mobilized. The "problem" is that the solution is still very slightly cloudy (I am told by soil experts from the Department of Soil and Geology of the University of La Laguna, which may be due to the amorphous clays in the soil). What do you think I can do to improve the laboratory experience without making it too complicated for the students?
Thank you very much
Dr. Felipe Hernández Luis
Departamento de Química
Universidad de La Laguna
Tenerife-España
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