18 November 2021 1 2K Report

I intend to characterize the fluorescence property of an unidentified fluorophore in a liquid culture. However, the analysis was interfered by the fluorescent flavins (predominantly riboflavin) produced by bacteria.

I learnt that C18 column can remove flavins from the liquid medium, but I found that it also removed the fluorophore I wanted to study.

Is it possible to specifically remove flavins? Thanks.

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