Hi all,
I'd like to FACS D1 and D2 MSNs from rat striatum with the aim of doing either qPCR or RNAseq. ASFAIK, all commercially available ab's are not worth a dime and for practical reasons I can't get my hands on the reporter mouse-lines out there. I was thinking whether using PrimeFlow to stain the two populations and then sort would do the trick. Anyone, who has used the particular technique or that have other ideas? Any input would be much appreciated.
Cheers!
Tau