I digested insert (in pJET) and pNIP40b (mycobacteriophage MS6-derived) vector with XbaI; then I transformed the ligation (ratio 1:1 and 1:3) and the linearized vector (control) in Stbl2. After incubation, I observed huge growing in both plates.
How to solve this problem?
Molecular cloning. Complementation strain. Tuberculosis.
Wong Ling Chie
Maybe you can dilute your transformant before spreading them to reduce the amount of growth/colonies so that the number of colonies will be in valid range/plate (30-300 colonies), after that you can recalculate back the transformation efficiency using the dilution factor
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