What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View

How to add randomness to a channel using Matlab simulations?

I have to simulate multipath fading channels and add randomness to it.

21 September 2018 6,377 2 View

What is the best method for analysing anthocyanins in plants ?

Anthocyanins in plant extracts

13 September 2015 1,366 4 View

Wat is the precursor for depositing Calcium using vacuum deposition for Ca Test of barrier permeation?

I need to prepare the setup for Ca test for WVTR measurement. I am not sure how to deposit calcium on my glass substrate . We don't have ALD.

02 October 2014 3,057 2 View

How long can one store bisulfite converted DNA and at what temp so that it does not affect the quality of pyrosequencing?

I used 4 month old stored DNA @ -20 deg C. I got good PCR bands but not good result for pyrosequencing. Would it help if I store @ - 80 deg C and make aliquots of that?

04 May 2014 8,606 3 View

What is the mechanism behind the fluorescence in ester compounds?

Does the ester group act as donor or acceptor?

26 January 2014 635 4 View

What kind of innovative services should be provided to promote the accessibility of products and services of public library by underprivileged person?

01 January 1970 4,548 3 View

Could you please recommend a cytoplasmic counterstain for immunocytochemistry?

I am using ICC to stain some nuclear proteins in muscle-derived stem cells. I'm currently using a HRP-conjugated secondary and DAB substrate (From Biogenex) I would like to avoid haematoxylin as a...

01 January 1970 4,694 3 View

Factor analysis in case of small sample size and no. of variables more than sample size

I am working on a small dataset with 22 observations and using 30 variables under eight dimensions to prepare an index. I am using factor analysis for the same. But the condition for factor...

01 January 1970 7,418 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View