Hi Guys,
I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when my product has been eluted down?
Right now, I don't want to use HPLC cause I have large quantity to purify.
Thank you.
V. N. Ravi Kishore V.
Choose sephadex with molecular weight cutoff smaller than the polymer or protein. This selection ensures that the small molecule can enter the pores and the large protein/polymer does not enter the pores. The larger polymer will elute out in the void volume. (Note: If the void volume is not known then one can determine it.) The small molecules will elute out later. Thus they can be separated.
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