8 Questions 6 Answers 0 Followers
Questions related from Maixian Liu
Hello everyone, Recently I was running trastuzumab on native (tris glycine) gel from bio-rad in tris glycine running buffer (No SDS) pH 8.3. However, I didn't get any band on the gel. I checked...
07 July 2025 5,629 2 View
Hi guys, I am now synthesizing antibody-drug conjugate (ADC) by first reducing the Ab with TCEP and then conjugating with maleimide-containing drug. When I run the SDS-Page for the ADC (4%-20%...
02 June 2025 3,720 2 View
Hi Guys, I need some info regarding UV-Vis spectrum and excitation spectrum. I understand excitation spectrum is scanning the excitation wavelengths by fixing one luminescence wavelength. And...
03 March 2018 1,211 3 View
Hi Guys, I am inserting a DNA fragment encoding a short peptide into the plasmid. I purchased the fragment with XhoI restriction site at start and the end. The restriction site of XhoI on plasmid...
24 April 2017 2,729 5 View
Hi Guys, I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when...
05 October 2016 5,583 1 View
Guys, I have a question. How to re-nature the protein after the denature from GdmCl or Urea. Just curious! Thank you!
30 September 2016 3,988 8 View
Hi guys, I am doing protein expression in BL21(DE3) and plan to use ompA signal for periplasmic protein translocation. I want to know that if it is OK to have a tag, say T7 or flag tag before ompA...
05 April 2016 2,149 3 View
Hi Guys, Wish someone could help me out of this. Right now I am going to have Dual luciferase transfection (Firefly and Renilla) and I want to have stable cell line that can express both of the...
02 February 2016 2,563 2 View
