Maixian Liu

6 Questions 5 Answers 0 Followers

Questions related from Maixian Liu

If the absorbance spectra and excitation spectra don't overlap, what info can we extract from it?

Hi Guys, I need some info regarding UV-Vis spectrum and excitation spectrum. I understand excitation spectrum is scanning the excitation wavelengths by fixing one luminescence wavelength. And...

03 March 2018 1,184 3 View

How to separate plasmid with different fragment orientation?

Hi Guys, I am inserting a DNA fragment encoding a short peptide into the plasmid. I purchased the fragment with XhoI restriction site at start and the end. The restriction site of XhoI on plasmid...

24 April 2017 2,699 5 View

How to separate proteins/polymers without color?

Hi Guys, I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when...

05 October 2016 5,551 1 View

How to renature proteins?

Guys, I have a question. How to re-nature the protein after the denature from GdmCl or Urea. Just curious! Thank you!

30 September 2016 3,951 8 View

Can Anyone Advise me on ompA signal peptide?

Hi guys, I am doing protein expression in BL21(DE3) and plan to use ompA signal for periplasmic protein translocation. I want to know that if it is OK to have a tag, say T7 or flag tag before ompA...

05 April 2016 2,121 3 View

Can anyone advise me on a dual Luciferase assay using stable cell line and lentiviral transfection?

Hi Guys, Wish someone could help me out of this. Right now I am going to have Dual luciferase transfection (Firefly and Renilla) and I want to have stable cell line that can express both of the...

02 February 2016 2,527 2 View