How to search miRNAs for a plant from its TSA or EST data?

More Biswajit Jena's questions See All

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Which filtration method to go for run off water from dirty solar panels to be used again?

We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...

28 July 2024 5,778 2 View

When making abraxane with Nab tech, why is 9:1 Chloroform&EtOH used as solvents?

Is it the best optimization ratio based on experiments?

22 July 2024 8,170 0 View

Can diamond be grown using molecular beam epitaxy?

22 July 2024 9,755 2 View

How can I use LabVIEW to control of an ethernet-based driver?

Hello everyone, I am new to LabVIEW, right now we need to control the movement of an ethernet-based stage drive. We got the sample code from a collaborator but their instrument is USB-based, so we...

11 July 2024 5,385 2 View

Systematic review meta-analysis paper?

Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...

10 July 2024 5,490 5 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Adding isopropanol directly to eluted RNA samples?

I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...

23 June 2024 2,576 4 View

HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

23 June 2024 3,446 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View

How many samples do I need, which agar and DNA sequencing technique is most preferable when carrying out microbial diversity in three water bodies?

I am planning to conduct a research project on the microbial diversity in three different water bodies and I would like to know the optimal number of times I should sample each water body to...

06 June 2024 9,946 1 View

I did whole genome sequence for a leishmania parasite I got the sequence of rDNA in stead of GDNA can anyone ?

my question about the sequence analysis I did whole genome sequence for a leishmania parasite I got the sequence of rDNA in stead of GDNA can anyone ?

30 May 2024 8,671 0 View

How can I identify an unknown DNA sequence & termination ?

Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you

26 May 2024 1,590 3 View