What is the application of copper electrodeposited on Graphite ?

As we know Graphite is a nonmetal and copper is a metal then why we electroplate copper on graphite? What is the application of copper electroplate on Graphite?

13 September 2020 2,696 6 View

How to set up a common annealing temperature for primers in PCR?

I have a primer set. Tm of Forward primer is 56.6 and that of Reverse primer is 63.5. Which common temperature I should use for PCR reaction to gett a good amplification?

13 October 2019 1,234 5 View

Gene amplification in one gene and not other using same cDNA?

I am working with two different genes of whitefly at the same time. Earlier both of the genes were successfully amplified in desired size. All the PCR components and temperature conditions are...

12 July 2019 5,708 4 View

Not able to amplify the gene segment which showed a positive result earlier. Tried three different protocol along with master mix still no result?

Working on insect diuresis gene.

03 April 2019 7,497 2 View

Can anyone please suggest me about the extractant to to extract bioavailable Ti from soil samples?

22 September 2016 1,794 5 View

Can anybody enlighten me about the outcome of Diabetes induction in HEK celllines?

Dear all, if you can share any experience of Diabetes induction in HEK cell lines? And to be sure of induction of Diabetes, what will be the major procedure?

01 January 1970 6,510 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View