Hi all,
We have done experiments (a timeseries, which we used to model expression profiles) with the CATMA array platform. We found a gene that looked quite interesting, and there were two probes for it on the array. However, the probes showed different expression profiles.
I thought it could be some unpublished splicing, so I designed qPCR primers on the areas covered by each of the probes. With both pairs I validated only the second probe, that unfortunately was not the one that was in our model.
So we think that there could be a mismatch between the probe and its ID on the microarray. The question is: is there a way to sequence that probe from the slide? It's likely that we can have access to some old CATMA slides.
Thanks for any input,
Ruben
I expect that you have already consulted someone in the group/company who supplied you with the microarray. If not, that may be first avenue to pursue. If there was an error in layout, possibly they may track it down and you could have a quick answer.
Striping the tiny spot and sequencing, though theoretically possible, it will be tedious to say the least. It may be easier to redo the probing on a new, correctly defined, microarray slide.
Best...
Hi Tausif, thanks for your answer. I contacted a rep from another company, since the CATMA consortium was dissolved a while ago, and when I tried to contact scientists from that consortium the emails bounced back.
I knew it could be a long shot, but I thought it was worth a go to try research gate.
Cheers,
Ruben
- Badges
- Science topic
- Similar topics
- Psychology
- Mental Processes
- Learning