How to remove residual adhesive glue from chamber slides?

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Should soluble fiber be considered a conditionally essential nutrient?

Carbohydrates are still considered a non-essential nutrient. But if we include dietary fiber, except in specific adaptive contexts of particular ecological niches, is this a reality today?

11 April 2024 6,566 0 View

How can I reduce the variability of CFU counts between replicates?

I'm challenging bacteria with different compounds, then plating the suspension on LB agar to assess CFU and understand the bactericidal properties of my compounds. However, I'm encountering...

10 April 2024 7,459 7 View

Which cell-penetrating peptides (CPPs) do you recommend to deliver intra-cellular cargo in chitin cell-walled unicellular eukaryotes?

Dear colleagues, I am working on a project in which I want to deliver oligonucleotides (specifically, molecular beacons (MBs)) inside different protist species. To do so, I am going to use...

18 March 2024 2,778 0 View

Which tool is the most reliable for predicting RNA secondary structure in oligonucleotides?

Dear colleagues, I am designing RNA molecular probes for FISH, and I want them to adopt a hairpin structure. I have checked for the possible secondary structure formation of some of the candidate...

14 February 2024 7,811 2 View

Can anyone suggest any interview questionnaire for leadership style?

Hello! I am currently conducting a qualitative study about leadership styles where I need to determine the leadership style used by my participants and I need help in looking for an already...

22 June 2023 7,753 3 View

Is it correct to analyze the Importance Value index for epiphytes?

I understand that Importance Value is a measure of how dominant a species is in a given forest area and It is a standard tool foresters use to inventory a forest. But does it have sense on...

06 June 2023 3,806 1 View

Does anybody know how to use thermo-calc to simulate De-P for converter slags(CaO-SiO2-MgO-FetO-(MnO-Al2O3-P2O5)?

My database is SLAG 4. I know this database has some limitations, but that's what I have.

21 August 2022 1,045 0 View

It is possible to calculate the content of amorphous ACn phases by the external standard method using the GSAS II?

I need to calculate the content of unquantified amorphous or crystalline phases in my sample. I did the analysis of a standard sample to apply the external standard method and I would like to know...

10 July 2022 3,129 1 View

Event-based inventories?

Hello! I'm trying to gather event-based inventories, or specific events that were mapped/inventoried of landslides, debris flows or flash floods around the world. Can you recommend any specific...

09 June 2022 5,648 3 View

Standarization samples for NIR portable devices?

I'm looking for suggestions to know which samples I could use as a standarization and model transfer for NIR portable devices. Right now we are using mixes of urea and glycerol with different...

09 March 2022 4,401 0 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Normality assumption for linear regression is The assumption of normality is whether for residual errors or predictor variavble?

When we conduct linear regression, there are several assumptions. The assumption of normality is whether the residual errors are normally distributed, not whether a predictor is normal?

31 July 2024 6,164 3 View

Is it possible to dock more than one drug in the same active site or pocket of a protein to study synergistic/antagonistic/additive properties?

I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...

28 July 2024 7,413 6 View

How apply Kao Residual Cointegration Test in eviews?

Kao's panel cointegration tests , is there anyone willing to explain me the eviews-9 output for the Kao's panel cointegration tests?

23 July 2024 5,051 4 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

Printing residues in an object in Pymol?

Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...

15 July 2024 1,727 1 View

How do I identify And count the number of active sites of an adsorbent?

Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.

10 July 2024 8,132 2 View

Hey there, What is The best recommended solvent( safe for use) to dissolve or remove Z-90 Adhesive from ceramic surfaces ?

as of my knowledge z90 is acrylic based sealant adhesive.

09 July 2024 8,660 4 View

I found trouble to run MD of my peptide with PTM in different force field. In Charm-GUI, it cant recognize the engineering residue. What can I do now?

I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...

05 July 2024 9,346 0 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View