I need lysis buffer composition for extracting the protein from coxsackie virus, which is grown in media containing FBS. How can we harvest the virus alone if we don't have ultracentrifuge?
Or maybe you can try PEG8000/NaCl precipitation. The precipitate can be easily spin down using bench-top centrifuge at MAX speed. Then you can resuspend the pellet using lysis buffer, or you can wash the pellet using 90% ethanol, then lysis buffer.