To format an input file for mutation analysis in MEGA X, you can follow the guidelines provided by the software. Typically, MEGA X accepts multiple sequence alignment files in various formats such as FASTA, NEXUS, MEGA, and PHYLIP.

you can format your input file in MEGA X using a FASTA format:

>Sequence1 ATCGGCTA... 3. Save the file: Save the multiple sequence alignment in FASTA format with a .fasta or .fa file extension.

Once you have prepared the input file in the appropriate format, you can then open it in MEGA X to perform more analysis.

HI thanks Pardis

I did try the way yo said but it did not work. Also, when i import the Alignment file (Mega) in MEGA for diagnose mutation, it shows following error:

Oh no! A failure occurred when validating the mutation data input file:

Invalid data at line: 1

Is there any different format for input file

Pls let me know

What multiple sequence alignment format(s) format did you try? Are you sure they were plain ASCII text and not opened with a text editor such as MS-WORD and accidentally saved in WORD (.doc or .docx) format?