Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

How to calculate trapping parameter of the deconvolution peaks?

28 July 2020 2,413 2 View

Have any professors ever done research about the expression of fusion proteins in Drosophila S2 cells?

1. How to scale up the expression of fusion proteins? 2. How to improve the purity of fusion protein to 90%? I have tried affinity chromatography, but the purity by SDS-PAGE was not satisfied....

04 November 2019 8,479 6 View

How to calculate the active area of CNTs modified glassy carbon electrode?

"The active area of so porous electrode as the authors used could not be correctly evaluated using the Randles-SEVCIK formula. Just because the depletion layers of active places may overlap." How...

28 October 2019 1,782 3 View

Future of bioinformatic analysis in plant science ?

please share your idea regarding the future of bioinformatic analysis in plant science? recommend the preferred PC and MAC programs for the beginner ,and for the advanced users? as well as any...

24 October 2019 8,814 4 View

Does anyone know about the AT3 cell line?

Recently I need to construct TNBC syngeneic mice model and my supervisor tells me to use AT3 cell line. But when I check the papers online I find that 2 papers say the AT3 is a prostatic cancer...

06 September 2018 7,174 2 View

Bad performance of RQ-PCR standard curve constructed by plasmid

Hi guys! Recently I encountered a problem about the Albumin (ALB) standard curve which was used for normalizing my samples. The ALB standard curve was constructed by serial dilutions of plasmid...

25 July 2017 8,762 3 View

Recommendation for instrument and method to produce the macro-structure of weld joints?

Detailed information of the equipment (i.e. name, company, price, working mechanism) is highly appreciated.

08 September 2016 6,402 0 View

Where to find the definitions of thin plate, medium thick plate and thick plate?

10 November 2015 3,459 0 View

How can I get the free GIS data of China?

16 May 2015 3,254 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View