I am using Thermo Scientific GeneJET gel extraction kit, we have no columns left but have additional reagents, now how can I regenerate them? I have read about the 1M HCl and 1M Phosphoric acid protocols, but one user (deleted ID) on researchgate have commented that 1M HCl cannot be used for regeneration of Gel Purification columns, so in your experience is it possible to regenerate my columns with 1M HCl? as I cannot find phosphoric acid in my lab or other labs within my department.
What is the difference in the membrane of Gel Purification and Nucleic acid purification columns? are both the membrane made of Silica resin only?
I can't answer you because I've never tried. However Phosphoric acid is also called orthophosphoric acid or phosphoric (V) acid.
Hello Shayan. If you are trying to extract DNA from agarose gel you can also do the following (no kit needed). Take a 1.5-mL centrifuge tube and poke a hole in the lid large enough to accomodate a 0.2-mL PCR tube. Poke a small hole, using a needle, at the bottom of the 0.2-mL tube. Place glass wool (preferably siliconized) at the bottom of the 0.2-mL tube. Place the 0.2-mL tube through the hole of the lid of the 1.5-mL tube. Now place your gel chunk (whole or chopped up) in the 0.2-mL tube and close the lid. Centrifuge the tubes 10-20,000 rcf for 20-40 minutes. Your DNA should be in the bottom of the 1.5-mL tube. Now just ethanol precipitate and resuspend with your desired solution. Good luck!
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