I am working on PPD-based hair dye. It leaves stains on the skin. What should I do so that it will not leave stains on the skin except the hair?
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...
30 July 2024 2,104 6 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...
23 July 2024 6,127 1 View
I am writing a paper as a part of my course. I am new in London and was wondering that what publications should look upto?
21 July 2024 3,538 1 View
After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...
18 July 2024 4,674 4 View
It has been a quite long time since the Russia and Ukraine war has been going on and if we see the context of Geopolitics and International Relations we don't see a mediation between the two...
18 July 2024 6,737 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View