We would like to study the mouse skin degranulation after chemical exposure. We are getting lot of dark brown color artifact in tissues irrespective of control or treatment group. Can anybody guide us to get rid of this problem?
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I am Ruthra, and I have been working as a psychologist for the past 10 years. Now, I'm interested in conducting research and I would like to conduct a survey on mental health and wellbeing among...
15 July 2024 4,119 4 View
DoDocking of the Target protien
14 June 2024 7,489 5 View
Can someone clarify whether the Pymol version is available in GitHub free or to be subscribed to? Can this Pymol be installed in Python and be used for research publications ? I installed Pymol...
12 June 2024 9,969 2 View
That is product of two consecutive integers is a Fibonacci number and hence it must be even.
24 March 2024 1,912 4 View
PhD University suggestions are welcome from across the world.
13 March 2024 1,508 7 View
Vector 1 (Insert 1, Insert 2, Insert a ) and Vector 2 ( Insert 1, Insert 2, Insert b) contain three inserts in which only the Insert a and b are different. These two vectors are constructed by...
22 January 2024 2,759 4 View
Hi all, I have the plot profile in imageJ and I saved the data (Distance_(microns) & Gray_Value) for the analysis. Could you please suggest to me how to do quantification with this? Thank you
03 December 2023 3,361 1 View
I want to cluster multiple pdb files with respect to one reference pdb. I have to use Align > all this to *Ca in Pymol. However, how could i do this via scripting in Pymol? As I have multiple...
15 October 2023 10,053 3 View
How to show two peptide structures side by side on Pymol. I have 100 PDB files. I want to show all the 3D image structures side side in a two-dimensional array form. How could I do the same in Pymol?
14 October 2023 8,195 6 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View