I was trying to recalculate the RIN value using the Agilent Bioanalyzer. because it couldn't calculate the RIN value due to 'unexpected ribosomal ratio' error flag. I checked the setpoint for the ribosomal ratio for RIN calculation and it was 0.7. I then edited the fragment size of the 28s and got a ratio of 1.1 but still the error says the same and no RIN value. So, does that mean it is impossible to recalculate the RIN value? Thanks in advance for your kind help.
I got the answer friends! It is all dependent on the setpoints under the RIN section. Changing the threshold to max. 1 recalculated the RIN value
I have the same issue with the bioanalyzer, where did you change the threshold to recalculate the RIN value, it only lets me modify the lower marker but the RIN stays the same. And besides, it doesn't detect the peak from the 28S, but the graphics do.
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