Using fluorescent dyes
Hi Simran
Have a look at the following web links on Springer protocols
http://www.springerprotocols.com/Pdf/doi/10.1385/0-89603-297-3:113?encCode=Q0lNOjMxMTozLTc5Mi0zMDY5OC0w&tokenString=8KNPHpDbdq13JkX4YiiNuA==
http://www.springerprotocols.com/Pdf/doi/10.1385/0-89603-297-3:107?encCode=Q0lNOjcwMTozLTc5Mi0zMDY5OC0w&tokenString=EQz6A34fP9JcUp+NdvBIQQ==
To my knowledge you can do quantitative analysis only for bacterial cells, for mold and fungi you can do only qualitative. Plating is the other alternate.
thank you Sir for your valuable suggestion
Hello everyone, I'm not able to do fatigue analysis of my model in ANSYS workbench, because I need some values .The material that I selected is ABS High Impact. I attach a file please check and...
19 September 2023 6,539 0 View
Presently, QGIS is also being used widely in various GIS projects, still companies are looking for experts in ArcGIS. Can we expect that this scenario will persists?
23 May 2021 203 30 View
Hi, I am new to research and having a difficult time understanding the meaning behind some things. I am working on an experimental PCR where we are doing SDM and mutating a plasmid with mutagenic...
04 April 2021 8,890 5 View
In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...
01 March 2021 2,442 4 View
I want to check antibacterial activity of CaCO3 nanoparticles, and I have to make suspension in which they can easily disperse. I have tried DMSO, chloroform, ethanol, saline and DI water, but...
04 November 2020 4,404 3 View
I was working on MIB server for educational purpose. But since a week I am unable to use the server. It is either restricted or down, I don't know what the problem is. But I want to dock my metal...
02 November 2020 9,006 3 View
Need to know sonication time for SEM measurements of nanoparticles
07 September 2020 4,643 3 View
I want to analytically calculate the number of activated dopant concentration in Mg-doped AlGaN when the doping concentration as well as the ionization energy level for the acceptor is known. If...
11 June 2020 1,166 0 View
I want to conjugate my antibody with oligo. I used SMCC approach. But the problem is that i get 3 types of antibody as product. 1 Unlabeled antibody 2 Labelled antibody with single chain of...
03 September 2019 7,281 3 View
I have a cell line that is stably expressing eGFP and mRFP1. I want to introduce another color that will be easily distinguishable by flow cytometry. I was suggested to use AmCyan which has an...
02 August 2019 878 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View