I am working with L serine esterfication reaction and I want to quantify the product L-serine Methyl ester using HPLC. Can anyone guide with HPLC Quantification?
Thank You for your reply sir. But can you please suggest me any method in GC or HPLC with out derivatization. I am using RPHPLC with UV detector.
You will need RID detector. It has no chromophore so RID is best detector for it. You can give try by setting wavelength on UV at 210 nm.
your compound is already in the methyl ester form, so it should be ready for analysis, all you need is to extract to GC compatilble solvent.
Of course GC technique is the most direct technique for analysis of the L-serine methyl rster.However if you need to develop RP-HPLC method for the analysis of this compound then you need to derivatize it to add a chromphore that render the compound UV senstive e.g forming N-Benzyloxycarbonyl-L-serine Methyl Ester then you can develop an HPLC method.
Thank You Hassan sir for your reply. Sir Can I kindly know whether direct analysis of L serine Methyl ester is possible with GC. I am using GC with Carbowax or HP5 columns with FID Detector. Sir what is the GC method for doing this directly.
Check the link mentioned in Dr.Narong Chamkassem reply it is very useful. again you may need derivatization to improve on the sensitivity.
Dear Sundeep can you please send the process ingredients and reaction procedure
Sir, I am synthesizing L serine Methyl ester using Lserine, Thionyl Chloride and Methanol. I want to analyse the final formed product in this case sir.
Sundeep,
L-Serine methyl ester purity will get by GC but quantification of L-serine by HPLC ( Derivatization method only), but in this reaction you have to quantify the L-serine only.
But in this process is 100% reaction will complete hence there is no possibility of L-serine content, so you better to go with GC method only.
Narasimha Rao Sir,
I want to quantify the reaction. So is it possible to quantify either L serine or the Methyl Ester Product formed by using GC. ( I am using GC with FID detector and Carbowax and HP5 columns.) Can you help me how to proceed sir.
Thank You
I thought your analyte from your experiment is the ester of L-serine per your question above. Review the paper I gave you see if you can extract into GC compatible solvent. First of all, you can inject standard of L-serine ester to see if you can see it at the level you want to see. If you cannot see low enough, then you have to do cleanup and concentration.
Sundeep,
Generally purity for solid compounds or high boiler compound, we should go with HP-1 or HP-5 columns since these column have capable of high temperature around 300°C.
- Badges
- Science method
- Similar topics
- Chemistry
- General Biochemistry
- Biochemical Techniques
- Analytical Chemistry