26 April 2019 4 3K Report

I am currently trying to encapsulate a lipophilic vitamin in SUV (DPPC+Cholesterol) with concentration of 0.75 : 1.26 : 0.14 mM consecutively. After sonication, I want to separate unincorporated vitamin by centrifugation.

I've tried:

1. 1,500 RCF ; 30 mins; 18°C

2. 6,000 RCF ; 30 mins; 18°C

3. 13,800 RCF; 30 mins; 18°C

4. 21,500 RCF; 15 mins; 10°C

but after all above, the system still homogenous (no sedimentation except a tiny black pellet that I thought to be titanium from sonication).

Is there any recommendation or any reference that I can look up to?

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