How to purify IgY antibody from egg?

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What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

New problem Statement on Solar Energy?

Dear All, I want to know the new research topic on Solar Energy for my PhD. work. Please let me know.

13 January 2020 4,388 1 View

What is the appropriate way / index of expressing dissimilarity of elements in a large sample collection?

Ex. I have taken a sample of x papers. Total number of contributing authors in these x papers are N authors. However, some authors have contributed in more than one papers in the sample. Say the...

03 July 2019 9,941 1 View

What is the lowest concentration you should take for determination of solubility of compounds by LC-MS method?

I have to determine solubility of LC-MS of completely new compound, however, the sample provided is very low. So, I would like to know what is the safe concentration to start.

07 February 2019 6,945 3 View

How to determine the connectivity of the pores through BET Isotherm analysis? How do we know the pores of the material are interconnected or isolated?

I have synthesized a series of porous organic polymers by tuning the linker length and observed a trend of variation of surface area (ultramicroporous, high surface area to mesoporous, low surface...

25 November 2018 3,962 2 View

Resuspend salivary proteins in 1X Coupling Buffer???

I have to enrich glycoproteins from salivary samples. I am using hydrazide resin. But it's very difficult to resuspend salivary proteins in 1X coupling buffer. Is there any solution for it?

07 September 2017 3,897 2 View

How to obtain ethical clearance for a multi-center study?

We are planning to conduct a questionnaire-based study among dental students of different dental colleges of Nepal (within a country). Should we obtain ethical clearance from each institution's...

03 October 2016 7,434 5 View

How to check titre of egg yolk antibody against bacteria?

How can I determine the titre of my egg yolk antibody against bacteria..or can anyone plz tell me how to check antibody titre against bacteria.thank you?

08 July 2016 3,224 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

C.A. (Kees) Kan

You might try the method described in this publication

Article Induction and Characterization of Multianalyte Antibodies Ag...

Chankit Giri

sir please send me thr publication

All papers uploaded by the author on Research Gate, like this publication, can be simply downloaded from it. No need of sending files.