How to purify IgY antibody from egg?

More Chankit Giri's questions See All

Is there any reliable method besides LC-MS for quantifiying Pepstatin A compound?

I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.

25 January 2024 3,341 0 View

How does technology dependence affect the development of metacognitive skills in learners?

Any thoughts on this?

23 October 2023 8,131 0 View

How to remove only water from a 6% ethanol, 4% lignin and 90% water mixture ?

can anyone help me out in the process for removing water from 6% ethanol, 4% lignin and 90% water mixture and retain ethanol and lignin alone.

27 September 2023 6,538 0 View

How to create the intermediate structures in CO2RR?

I am exploring the CO2RR pathway. While doing so i need to know the intermediate structures. How to create those intermediate structures??

13 August 2023 4,812 0 View

How to calculate Plasma Frequency using VASP?

I started with structure Optimization and then ran SCF calculation to Generate WAVECAR file. Then calculated Plasma frequency using LOPTICS=.TRUE. and following tags. is the approach correct for...

07 August 2023 7,832 1 View

Is there any specific method for purification of folic acid from bacterial culture?

After extraction of folic acid from probiotic bacteria is there any further purification step?

15 June 2023 6,235 6 View

I am in the middle of research on lignin ethanol mixture , I have hit a road block with water separation from lignin ethanol mixture; please suggest?

I have evolved a lignin/ ethanol/ water mixture with a ratio of 10:15:75, I am facing challenge to remove water from this mixture; pls suggest .

01 June 2023 824 0 View

How to measure damping coefficient for multiple modes of beam vibration from the impact hammer experiment?

From the impact hammer test, I can able to obtain multiple natural frequencies of a cantilever beam. Now, to obtain the material damping coefficient of various modes of vibration, I believe that...

28 May 2023 5,345 4 View

Protein is precipitating after purification, what should i to avoid it?

Elution buffer composition is- 50mM NaH2Po4, 300mM NaCl, 250mM immidazole at pH 8. PI of my protein is 6.7. I purified My protein using Ni-NTA resin and run sds page and till the completion of gel...

23 February 2023 4,678 12 View

How to use imperfection in Abaqus for Non linear Analysis?

Respected all; In Abaqus software, I am running Non-linear Analysis on Steel beams like corrugation and castellation. For doing so, I have noticed on youtube of use some editing in Edit...

05 December 2022 4,636 7 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

C.A. (Kees) Kan

You might try the method described in this publication

Article Induction and Characterization of Multianalyte Antibodies Ag...

Chankit Giri

sir please send me thr publication

All papers uploaded by the author on Research Gate, like this publication, can be simply downloaded from it. No need of sending files.