High density lipoprotein and keratinocytes??

27 February 2019 0 5K Report

hi their,

i preparing to do cell proliferation assay for keratinocytes treated with different HDL concentration. do any one can help me with what is the best HDL concentrations to use and if their are papers done it before.

thanks

More Ka Bs's questions See All

How do I perform data analysis in Excel on two variables with varying amount of questions and values?

I am studying leadership style's impact on job satisfaction. in the data collection instrument, there are 13 questions on leadership style divided into a couple of leadership styles. on the other...

16 April 2024 4,784 3 View

Why am I continuously getting positive signal in my 3 plex negative control in the RNAscope multiplex V2 kit?

I am currently working to optimise RNAscope multiplex V2. I have troubleshooted the issue with several tests. I have washed thoroughly with wash buffer even with their recommended guidelines of...

29 February 2024 4,764 0 View

How to immobilise alpha amalyse enzyme in mesoporous silica?

functionalized mesoporous silica unable to immobilise alpha amalyse enzyme

21 February 2024 4,895 1 View

How can I improve the DNA yield of my sample (hermit crabs)?

I am using the High Pure PCR Template Preparation Kit (Roche) to extract DNA from hermit crabs. These samples are stored in 99% ethanol at room temperature. I have found that many of them exhibit...

28 November 2023 3,989 1 View

Which M and SD should I use for calculating Z-score for a pre-post test RCT design?

I have two datasets (both with experimental group and control group) which measure the same construct with two different forms due to age differences. All groups completed the measure at a pretest...

08 November 2023 6,667 1 View

If I could not add triton X-100 for my immunostaining, what should I do?

I am currently doing an experiment related to nanoplastics. Recently, I did an immunostaining experiment with triton X-100 to permeabilize the cells, I found out that the nanoplastics signal...

26 October 2023 7,218 0 View

Is it possible to conduct data analysis on a completely anonymous pre-post Perceived Stress Scale survey?

Hi all! I designed a Wellness Program for Nurses as my QI project to evaluate the impact of this intervention on the participant's stress levels (using Perceived Stress Scale). I am planning to...

27 September 2023 3,438 4 View

How to perform Bland-Altman analysis on two AST methods with large range of MICs?

Hello fellow researchers. This is my first time asking question on ResearchGate, and I would be grateful if someone could provide me with suggestions and help. There are two questions that I want...

25 September 2023 5,037 0 View

How to publish your phd thesis?

How and where to publish phd these?

21 May 2023 3,981 4 View

BOTworld results cannot read by Leonardo, Envi-met v3.1?

Hello everyone, Does anyone have the Envi-met software (e.g. in earlier years) which could read the results of BOTworld in Envi-met? I am new for the BOTworld and have successfully gotten some...

22 April 2023 1,535 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View