Your question is not completely clear to me, would you be able to clarify what you exactly want to know?

Do you want to isolate A. marginale from blood and thus lysate the infected erythrocytes to purify A. marginale?

Dear Arunraj M. R 

DNA was extracted from whole blood using the High Pure PCR Template

Preparation Kit (Roche) following the manufacturer’s instructions. Briefly, 200 μl of

 blood was mixed with 200 μl binding buffer and 40 μl proteinase K, incubated for 10

 minute at 70 ◦C. Added 100 μl of isopropanol and sample loading onto a column. This was followed by successive washing, then collect DNA in a final volume of 100 μl. 

Feel free to contact me at any time if you has any questions in the protocol 

if what you want is to purify the initial bodies form infected blood I can send you our protocol which includes freeze-thaw, hipotonic lisis, sonication and gradient centrifugation to obtain 99.99 membrane-free initial bodies.

Please let me know

sorry for the confusing question. I just want to purify the initial bodies from infected blood. 

Arunraj Hi

Let translate my protocol to english and will send ASAP