AMBER tleap solvatebox always undervalues the density, why?

I've been searching for an answer to this question for years, and I'm shocked I haven't found any discussion on any forums. Here is an example to provide more details: I am preparing a 450...

20 January 2021 7,561 2 View

Are there available scales or questionnaires to measure change management?

Seeking to connect with authors who may want to share their scales or questionnaires to measure change management.

27 September 2020 374 6 View

How do I combine two variables into one in SPSS?

I have a data set with information on BMI which is derived either from self-reported (variable 1) or measured weight (variable 2). Each subject has information on BMI in one of these variables,...

07 October 2019 431 3 View

How to export ANSYS Static Structural results to a format that Paraview can read?

My ANSYS model gives me .rst and .dat output files and Paraview gives me errors when I open them. How to get ANSYS to export a format that Paraview can successfully read? Thank you :)

18 March 2019 1,481 1 View

One-Class Classification with support to HDF5?

Hello, I need to perform a One-Class Classification (OCC) task, but the training data does not fit on my memory. I was using sklearn's OneClassSVM and when I realized that I would need online...

04 November 2018 289 4 View

Statistical differences between SPM and FreeSurfer?

Hi, Does anyone know what are the statistical differences between SPM and FreeSurfer? Thanks!

19 September 2017 3,360 5 View

How to remove protein from DNA mini prep?

17 October 2016 3,050 6 View

Is plasma treatment comparable to corona treatment to prepare a thermoplastic surface for bonding with an adhesive?

30 August 2016 4,951 4 View

What software is now being used in place of KC Junior for BioTek platereaders

We used to use KC Junior to run our Elisas and XTT assays as well as our luminometer. Having installed new computers on our plate readers we now are informed that KC Junior is no longer available...

14 April 2016 1,190 3 View

How effective is the restriction digestion step for TRF telomere length measurement?

I read that after HinfI and RsaI digestion the non-telomeric fragments are typically

01 March 2015 5,923 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View