I am struggling at finding a suitable approach for extracting soybean seed’s storage protein fractions, i.e., albumin, globulin, prolamin, and glutelin, and their subsequent determination through UV-Vis Spectroscopy using Bradford staining solution and bovine serum albumin (BSA) as standard.
As the obtained results were significantly different than those found in the literature, I came across some major issues:
1) Is the sequential extraction (water, sodium chloride 5%, ethanol 60%, and NaOH 0.4%) method suitable for extracting the albumin, globulin, prolamin, and glutelin fractions of soybean seeds singly?
2) Is the BSA an adequate standard for determining either globulin, prolamin, or glutelin fractions?
Thanks a lot!
Dear @Gabriel Sgarbiero Montanha I have attached four PDFs of papers published in reputed journals; hope, these would be useful to you.
Best wishes, AKC
The methodology is properly articulated in the attached documents. Article Soybean Mutants Lacking Abundant Seed Storage Proteins Are I...
Article Differential Accumulation of Soybean Seed Storage Protein Su...
Hi. I hope the following website could help you:
https://patents.google.com/patent/US4704289A/en
Check https://onlinelibrary.wiley.com/doi/am-pdf/10.1002/jsfa.9235
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