We hardly try to produce foam cells in vitro with oxidized LDL. We use human LDL and try to oxidize with CuSO4. But this doesn't work. Is there anybody with a working protocol ?
Do you isolate LDL from blood? how do you resuspend them and what concentration of CuSO4 do you use?
Hello everybody ! Who can tell me how to produce platelet rich plasma as simple as possible (a working protocoll would be nice). Thanx in advance, Atilla
07 June 2022 5,870 2 View
Hello, I am a doctoral student and currently working on a mediation model where X is the treatment, M is the mediator and Y is the outcome variables. I also use Z as the single instrumental...
12 February 2021 4,654 7 View
Hi, It is possible bulk density decrease with depth ? (0-15 and 15-30 cm)
09 June 2020 5,177 15 View
I am interested in using SoySNP6k panel for a specific soybean project. In order to check the usefulness of this approach, I need to SNP information for this Panel. If possible, Could you please...
15 November 2019 3,997 2 View
Is the blinding (beneath the foundations) considered as important layer? And how many the optimum thickness of blinding (in Iraq between 5-10)cm.
20 December 2018 5,308 3 View
On one hand one can understand time dilation related to gravitational potential difference by considering the uniform acceleration as an infinitesimal change of velocity thus as an infinitesimal...
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How much daily water consumption per person in the countries of the world
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effect of flood hazard below reservoirs that are impounded by earth fill dams in a hilly topography
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Apart from economic frameworks like oracle or sql server, I am looking for academic business intelligence data to conduct some experimental researches such as applying data mining...
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14 June 2015 6,435 10 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,032 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,105 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View