How to produce DNA coated gold nanoparticles for gene delivery? What kind of ligand can I use for coating nanoparticles?

01 January 2013 1 3K Report

You could try looking at the following publication (PMID: 21663596). They used a similar technique (spermidine/CaCl2) as is used to coat gold microcarriers with DNA.

BMC Biotechnol. 2011 Jun 10;11:66. doi: 10.1186/1472-6750-11-66.

Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles.

O'Brien JA, Lummis SC.

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