I want to extract TEM or blaTEM gene of E Coli for QPCR. But first I have to get the sequence of them gene followed by primer design and then In Silico PCR after that.
Can someone tell me
where to search for the sequence of AMR gene Ensemble or NCBI?
Which tool I use for Primer design or should I do it manually?
Firstly, you only need qPCR if you are trying to determine the copy number compared to a single copy chromosomal gene, regular PCR would be good enough for presence/absence detection. I would recommend checking GenBank for the sequence of your genes. Primer Blast can do some automatic primer design but if that yields no good options you can always do it manually and then use the Multiple Primer Analyzer tool on the ThermoFisher Scientific website to check for problems.