I want to extract TEM or blaTEM gene of E Coli for QPCR. But first I have to get the sequence of them gene followed by primer design and then In Silico PCR after that.

Can someone tell me

  • where to search for the sequence of AMR gene Ensemble or NCBI?
  • Which tool I use for Primer design or should I do it manually?
  • Which tool I use for in silico qPCR?
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